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* 1. AIN-93 was originally compared against a chow diet. What control diet should the new formula be compared against?

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* 2. AIN-93G was originally tested in young outbred Sprague-Dawley rats and outbred Swiss-Webster mice of both sexes. Should the new formula be tested on the same or different strains? Keep in mind that inbred strains may give non-representative results.

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* 3. Several micronutrients for pregnant mice are less than the suggested NRC requirements and there is evidence for low iron in lactating rats. Should the AIN-93G diet be recommended for pregnant/lactating animals?

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* 4. For AIN-93, the primary endpoints were growth, kidney calcification, and bone mineralization. What endpoints should be used for testing the new diet?

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* 5. Carbohydrate in AIN-93G is 10% sucrose, ~40% cornstarch, and ~13% dextrinized cornstarch. Sucrose is important in pelleting. Should the carbohydrate composition be:

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* 6. Fat in AIN-93 is soybean oil but it provides excess linoleic acid (18:2, n-6) and currently less linolenic acid (18:3, n-3) than soybean strains grown in 1993. Should we:

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* 7. Fiber in AIN-93 is 5% cellulose; many chow diets have 3-4 times that amount. Given increased knowledge of the intestinal microbiome, addition of fermentable fiber is proposed.

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* 8. Other factors you consider important to consider:

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* 9. Your name/affiliation (optional):

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