Sequencing Survey Question Title * 1. How is NGS currently being used? It is a necessary and integral part of our workflows It is ancillary, but regularly used (library QC, confirmation sequencing, etc.) We don’t currently use it, but we could (replace qPCR or Sanger, introduce as a QC step, etc.) We don’t currently use it, but we have some cool ideas on how we might be able to We don’t use it and don’t have a need for it Other (please specify) OK Question Title * 2. If you are using NGS, how much are you sequencing? Big projects – NovaSeq/HiSeq/NextSeq runs (400M – 6B reads) Medium-sized projects (25M – 400M reads) Small projects (4M – 25M reads) Tiny projects (1M – 4M reads) Not at all Other (please specify) OK Question Title * 3. How often do you sequence on average? Multiple times per week Once per week Once per month A handful of times per year Never OK Question Title * 4. Do you tend to batch samples over time before submitting a run? No, our throughput is consistent enough that we don’t wait long to batch samples. Yes, we tend to wait for a period of time before sending out for sequencing. It varies - sometimes we batch for higher throughput runs, sometimes we’ll only send out a few samples at a time. We don’t currently use NGS. OK Question Title * 5. What potential high-throughput applications are of interest to you? (can select multiple) WGS (human) Exome Seq Shotgun Metagenomics Methylation Bulk RNA-Seq Single Cell RNA-Seq Other (please specify) OK Question Title * 6. What potential low-throughput applications are of interest to you? (can select multiple) WGS (small genome) Microbial (WGS, shallow shotgun, 16s) Targeted DNA (amplicon, enrichment, etc.) Targeted RNA (amplicon, enrichment, etc.) Library QC before sending out for high throughput runs CRISPR Screening Antibody/Phage Display Screening Other (please specify) OK Question Title * 7. If you are currently sequencing, what are some issues that you're facing? Cost Turn-around time Flexibility and control over experiments Ancillary steps (library prep or data analysis) We don’t currently sequence OK Question Title * 8. If you don’t currently sequence, why not? (can select multiple) Cost Turn-around time Ancillary steps (library prep or data analysis) are too complicated We just haven’t started yet – we’re working on it! We want to, we just aren’t sure where to begin. We have absolutely no need for NGS. Other (please specify) OK Question Title * 9. If a benchtop platform was available for you to use, would you use it? Yes! Yes, but I’ll need some assistance on how to use it (library prep/analysis training, workflow consultations, etc.). Possibly – I’d like to meet with someone to discuss how we might be able to! No, but maybe in the future. Not now, not ever. OK Question Title * 10. How comfortable are you with NGS workflows (library prep, sequencing, and analysis)? I’m a pro! I’ve done it in the past and can figure it out on my own. There are some aspects I’m comfortable with and portions I may need training on. I don’t know how, but I’d love to learn because we’re interested in using it! I’m not going to use this and don’t need to know how. OK Question Title * 11. Please state your name and company OK DONE
