Flow Cytometry Instrument and Fluorophore Profile Survey Question Title * 1. In which country are you based? Question Title * 2. Which best describes your institution? Charity / Endowed Research Organisation Government Research Organisation Hospital / Clinical Center Industry (Contract Services) Industry (Pharmaceutical) Industry (Reagent / Equipment Manufacturer) Private Research Organisation University Other (please specify) Question Title * 3. Are you a Core Flow Cytometry Facility? Yes No Question Title * 4. How many Users do you have (approximately)? Question Title * 5. Which instrument(s) do you use (select all that apply)? Amnis ImageStream Apogee A40 /A50 Attune™ Acoustic Focusing Cytometer BD Accuri® C6 BD FACSAria BD FACSAria II BD FACSAria II SORP BD FACSAria III BD FACSArray BD FACSCalibur BD FACScan BD FACScan / Cytek DxP8 BD FACSCanto BD FACSCanto II BD FACSJazz™ BD FACSort BD FACSVantage BD FACSVerse™ BD Influx Cell Sorter BD LSRFortessa BD LSRII Beckman Coulter Cell Lab Quanta SC Beckman Coulter CyAn ADP Analyzer Beckman Coulter EPICS Altra Beckman Coulter EPICS XL Beckman Coulter FC500 MCL Beckman Coulter FC500 MPL Beckman Coulter Gallios Beckman Coulter MoFlo XDP Beckman Coulter Navios Beckman Coulter XL-MCL BIO-RAD S3 cell sorter Compucyte iCys Cyntellect Celigo™ Cytometer Handyem HPC-100 iCyt Eclipse iCyt Reflection iCyt Synergy Millipore Guava easyCyte 5HT Millipore Guava easyCyte 6HT Millipore Guava easyCyte 6HT/2L Millipore Guava easyCyte 8HT Millipore Guava PCA Systems Miltenyi MACSQuant Analyzer Miltenyi MACSQuant VYB Partec CyFlow Cube Partec CyFlow ML Partec CyFlow SL Partec CyFlow space Stratedigm S1000 Stratedigm S1000EX Stratedigm SE500 Other (please specify) - add each instrument on a separate line Question Title * 6. Which fluorophores do you use? (select all that apply) 5-FAM 7C Alexa Fluor® 350 Alexa Fluor® 405 Alexa Fluor® 430 Alexa Fluor® 488 Alexa Fluor® 514 Alexa Fluor® 532 Alexa Fluor® 546 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 610 Alexa Fluor® 610–PE Alexa Fluor® 633 Alexa Fluor® 635 Alexa Fluor® 647 Alexa Fluor® 647–PE Alexa Fluor® 660 Alexa Fluor® 680 Alexa Fluor® 680-PE Alexa Fluor® 680–APC Alexa Fluor® 700 Alexa Fluor® 700–APC Alexa Fluor® 750 AMCA (Aminomethylcoumarin) AmCyan APC APC-Alexa Fluor® 680 APC-Alexa Fluor® 700 APC-Alexa Fluor® 750 APC-C750 APC-Cy5.5™ APC-Cy5™ APC-Cy7™ APC-eFluor® 780 APC-H7 Atto 390 Atto 425 Atto 465 Atto 488 Atto 520 Atto 532 Atto 550 Atto 565 Atto 590 Atto 594 Atto 610 Atto 620 Atto 633 Atto 635 Atto 647 Atto 655 Atto 680 Atto 700 BD Cy-Chrome™ BD Horizon™ PE-CF594 BD Horizon™ V450 BD Horizon™ V500 Brilliant Violet 421™ (BV421™) Brilliant Violet 510™ (BV510™) Brilliant Violet 570™ (BV570™) Brilliant Violet 605™ (BV605™) Brilliant Violet 650™ (BV650™) Brilliant Violet 711™ (BV711™) Brilliant Violet 785™ (BV785™) Brilliant Violet™ 786 (BV786™) Cascade Blue® Cascade Yellow® Cy2™ Cy3.5™ Cy3™ Cy5.5™ Cy5™ Cy7™ DyeMer™ 488/605 DyeMer™ 488/615 DyeMer™ 488/630 DyLight® 350 DyLight® 405 DyLight® 405LS DyLight® 488 DyLight® 547 DyLight® 549 DyLight® 550 DyLight® 590 DyLight® 594 DyLight® 633 DyLight® 647 DyLight® 649 DyLight® 650 DyLight® 680 DyLight® 750 DyLight® 755 DyLight® 800 Dyomics 547 (DY-547) Dyomics 549 (DY-549) Dyomics 590 (DY-590) Dyomics 647 (DY-647) eFluor® 450 eFluor® 565NC eFluor® 570 eFluor® 585NC eFluor® 605NC eFluor® 615 eFluor® 625NC eFluor® 650NC eFluor® 660 EviTag® quantum dots - Adirondack Green (520 nm) EviTag® quantum dots - Birch Yellow (580 nm) EviTag® quantum dots - Catskill Green (540 nm) EviTag® quantum dots - Fort Orange (600 nm) EviTag® quantum dots - Hops Yellow (560 nm) EviTag® quantum dots - Lake Placid Blue (490 nm) FAM™ FITC Fluorescein Fluorescein-5-maleimide (FMLA) HiLyte Fluor™ 488 HiLyte Fluor™ 555 HiLyte Fluor™ 647 HiLyte Fluor™ 680 HiLyte Fluor™ 750 IRDye® 680LT IRDye® 680RD IRDye® 700DX IRDye® 800 IRDye® 800CW Krome Orange™ Lissamine Rhodamine Marina Blue® mCherry Orange Cytognos 515™ (OC-515) Pacific Blue™ Pacific Green™ Pacific Orange™ PE PE-Alexa Fluor™ 610 PE-Alexa Fluor™ 647 PE-Alexa Fluor™ 680 PE-Alexa Fluor™ 700 PE-Alexa Fluor™ 750 PE-Atto 594 PE-Cy5.5™ (PC5.5) PE-Cy5™ (PC5) PE-Cy6™ (PC6) PE-Cy7™ (PC7) PE-Dylight® 590 PE-Dylight® 647 PE-Dyomics 590 PE-Dyomics 647 PE-Texas Red (ECD) PerCP PerCP-Cy5.5™ PerCP-eFluor® 710 Phalloidin, CF405M Qdot® 525 Qdot® 565 Qdot® 585 Qdot® 605 Qdot® 625 Qdot® 655 Qdot® 705 Qdot® 800 Quantum Red R-Phycocyanin (R-PC) RD1 (Phycoerythrin) Rhodamine Rhodamine Green™ Rhodamine Red-X Rhodamine Red™ s488-NHS Ester SpectralRed™ SureLight® P1 SureLight® P3 SureLight® PBXL-3 Texas Red TRI-COLOR® TRITC Other (please specify) - add each fluorophore on a separate line Question Title * 7. What size panels do you use? (select all that apply) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Question Title * 8. What problems are you encountering when designing panels - how do you think that these could be solved? (optional) Question Title * 9. Which Panel Design Tools do you use? (select all that apply) Beckman Coulter BioLegend Chromocyte eBiosciences Fluorish Other (please specify) - add each on a separate line Question Title * 10. Which other stains / dyes do you use? (select all that apply) 7-AAD CFSE DAPI DRAQ5™ (DNA dye) DRAQ7™ (DNA dye) DyeCycle™ Green Fluo-3 or Fluo-4 Fura Red™ Green Fuorescent Protein (GFP) LDS 751 (nucleic acid stain) LIVE/DEAD® Fixable Aqua LIVE/DEAD® Fixable Blue LIVE/DEAD® Fixable Green LIVE/DEAD® Fixable Red LIVE/DEAD® Fixable Violet mCherry MitoProbe™ DiIC1(5) pHrodo™ dye Propidium Iodide (PI) Red Fluorescent Protein (RFP) Rhodamine 123 SNARF® SYTOX® AADvanced™ Dead Cell Stain SYTOX® AADvanced™ Dead Cell Stain SYTOX® Blue Dead Cell Stain SYTOX® Green Dead Cell Stain SYTOX® Orange Dead Cell Stain TO-PRO®-3 YO-PRO®-1 Other (please specify) - add each dye on a separate line Question Title * 11. How do you find your antibodies? (select all that apply) 1DegreeBio Antibodies Online Antibody Directory Antibody Resource Antibodypedia Ask Jeeves Bing BioBrea Biocompare Chromocyte CiteAb Google Labome Linscott's Directory Manufacturer / Supplier website Other (please specify) - add each response on a separate line Question Title * 12. Who are your main antibody suppliers? Please add each response on a separate line Question Title * 13. Please Leave Comments - we are particularly keen to hear about any issues / problems that you are experiencing and how these could be solved.Many thanks for taking the time to povide us wth this information. Question Title * 14. Please leave your details if you would like to be kept informed on Chromocyte and our activities (optional) Name: Company: Address 1: Address 2: City/Town: State/Province: ZIP/Postal Code: Country: Email Address: Phone Number: Done