Sample Analysis using Microscale Thermophoresis on the Monolith |
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Here's What You'll Need to Bring
With Microscale Thermophoresis, you have a choice of a labeled or label-free assay.
If you'd like to do a labeled assay, please bring:
If you'd like to do a labeled assay, please bring:
- 100 µL of your protein at a concentration of 5-20 µM. It will be labeled on-site.
- 30 µL of your non-fluorescent ligand (interaction partner). It will be used at a concentration of at least 40X the Kd.
- 50 mL of your reaction buffer
- 200 µL of your fluorescent interaction partner. It will be used at a concentration below the Kd.
- OPTIONAL: If you already have labeled material, please contact us to discuss (michelle.pak@nanotemper-technologies.com). You'll need 200 µL of your fluorescent interaction partner. It will be used at a concentration below the Kd.
If you'd like to do a label-free assay, please bring:
- 100 µL of your protein at a concentration of 5-20 µM
- 30 µL of your non-fluorescent ligand (interaction partner). It will be used at a concentration of at least 40X the Kd.
- 50 mL of your reaction buffer
Feel free to generically label your samples (e.g. X, Y, Z) if you'd like to keep them confidential.