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Sequencing Survey
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1.
How is NGS currently being used?
(Required.)
It is a necessary and integral part of our workflows
It is ancillary, but regularly used (library QC, confirmation sequencing, etc.)
We don’t currently use it, but we could (replace qPCR or Sanger, introduce as a QC step, etc.)
We don’t currently use it, but we have some cool ideas on how we might be able to
We don’t use it and don’t have a need for it
Other (please specify)
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2.
If you are using NGS, how much are you sequencing?
(Required.)
Big projects – NovaSeq/HiSeq/NextSeq runs (400M – 6B reads)
Medium-sized projects (25M – 400M reads)
Small projects (4M – 25M reads)
Tiny projects (1M – 4M reads)
Not at all
Other (please specify)
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3.
How often do you sequence on average?
(Required.)
Multiple times per week
Once per week
Once per month
A handful of times per year
Never
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4.
Do you tend to batch samples over time before submitting a run?
(Required.)
No, our throughput is consistent enough that we don’t wait long to batch samples.
Yes, we tend to wait for a period of time before sending out for sequencing.
It varies - sometimes we batch for higher throughput runs, sometimes we’ll only send out a few samples at a time.
We don’t currently use NGS.
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5.
What potential high-throughput applications are of interest to you? (can select multiple)
(Required.)
WGS (human)
Exome Seq
Shotgun Metagenomics
Methylation
Bulk RNA-Seq
Single Cell RNA-Seq
Other (please specify)
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6.
What potential low-throughput applications are of interest to you? (can select multiple)
(Required.)
WGS (small genome)
Microbial (WGS, shallow shotgun, 16s)
Targeted DNA (amplicon, enrichment, etc.)
Targeted RNA (amplicon, enrichment, etc.)
Library QC before sending out for high throughput runs
CRISPR Screening
Antibody/Phage Display Screening
Other (please specify)
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7.
If you are currently sequencing, what are some issues that you're facing?
(Required.)
Cost
Turn-around time
Flexibility and control over experiments
Ancillary steps (library prep or data analysis)
We don’t currently sequence
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8.
If you don’t currently sequence, why not? (can select multiple)
(Required.)
Cost
Turn-around time
Ancillary steps (library prep or data analysis) are too complicated
We just haven’t started yet – we’re working on it!
We want to, we just aren’t sure where to begin.
We have absolutely no need for NGS.
Other (please specify)
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9.
If a benchtop platform was available for you to use, would you use it?
(Required.)
Yes!
Yes, but I’ll need some assistance on how to use it (library prep/analysis training, workflow consultations, etc.).
Possibly – I’d like to meet with someone to discuss how we might be able to!
No, but maybe in the future.
Not now, not ever.
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10.
How comfortable are you with NGS workflows (library prep, sequencing, and analysis)?
(Required.)
I’m a pro!
I’ve done it in the past and can figure it out on my own.
There are some aspects I’m comfortable with and portions I may need training on.
I don’t know how, but I’d love to learn because we’re interested in using it!
I’m not going to use this and don’t need to know how.
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11.
Please state your name and company
(Required.)
Current Progress,
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