SWI Science Data Collection Summary

Thank you for taking the time to contribute the science outputs data from your lab. We understand that each SWIPI implements the lab in his/her own way and may not use the same tester strains, perform extractions, or determine chemical structures. Please skip any sections that are not pertinent to your lab.

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1. Class Information (Required.)

Name of Institution

Name of Instructor

Semester/Term

Year

# of students with data reported in this segment

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2. General Metrics: Isolates

# of bacterial isolates picked

# of isolates tested for antibiotic activity

# of isolates positive for antibiotic activity

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3. Spectrum of Activity – # of isolates with activity against each strain/# of isolates tested against each strain (For example, if you test 10 isolates for activity against a particular strain and 2 show activity, you should enter 2/10.) Please note that "+" indicates the strain is Gram-positive strain and "-" indicates the strain is Gram-negative. This information is provided to help with subsequent questions.

Enterococcus raffinosus (+)

Bacillus subtilis (+)

Staphylococcus epidermidis (+)

Mycobacterium smegmatis (+)

Acinetobacter baylyi (-)

Escherichia coli (-)

Erwinia carotovora (-)

Enterobacter aerogenes (-)

Pseudomonas putida (-)

Other (please indicate)

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4. Gram-Negative & Gram-Positive Activity

# of isolates positive for only Gram-positive activity

# of isolates positive for only Gram-negative activity

# of isolates positive for both Gram-positive and Gram-negative activity

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5. # of isolates with positive activity against "n" tester strains (For example, if you test 2 isolates against 1 tester strain and they both have positive activity against both strains, you would enter 2 in the first box.)

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6. Sequencing

# of isolates sent for 16S rRNA sequencing

# of isolates with definitive sequencing results

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7. Extracts

# of isolates where extraction attempted

# of extracts produced in sufficient quantity for antibiotic testing

# of extracts tested for antibiotic activity

# of extracts confirmed to reproduce antibiotic activity

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8. Chemical Structure

# of chemical structures determined

# of novel chemical structures

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9. Additional Assay Summaries from Your Class (Two examples are included below.)

# of isolates with hemolytic activity

# of isolates with eukaryotic activity (specify anti-fungal, lethal to C. elegans, etc.)

Other (please specify)

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* 1. Class Information (Required.)

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* 2. General Metrics: Isolates

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* 4. Gram-Negative & Gram-Positive Activity

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* 5. # of isolates with positive activity against "n" tester strains (For example, if you test 2 isolates against 1 tester strain and they both have positive activity against both strains, you would enter 2 in the first box.)

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* 6. Sequencing

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* 7. Extracts

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* 8. Chemical Structure

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* 9. Additional Assay Summaries from Your Class (Two examples are included below.)

1. Class Information (Required.)

2. General Metrics: Isolates

4. Gram-Negative & Gram-Positive Activity

5. # of isolates with positive activity against "n" tester strains (For example, if you test 2 isolates against 1 tester strain and they both have positive activity against both strains, you would enter 2 in the first box.)

6. Sequencing

7. Extracts

8. Chemical Structure

9. Additional Assay Summaries from Your Class (Two examples are included below.)