We're excited you're bringing samples!

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nanoDSF Assays Can Be Set up a Number of Ways

We can measure:
  • The Tm of your protein in your buffer of choice. Different proteins and protein variants can be analyzed in parallel.
  • The Tm of your protein in different solution conditions. Different buffers, additives, detergents, etc. can be analyzed in parallel.

Here's What You'll Need to Bring

  • 30 µL of your protein at 1 - 300 mg/mL. Your protein should contain at least one tryptophan (in any buffer) per unfolding curve.
Please note: the protein concentration may be adjusted anywhere from 0.01 to 300 mg/mL depending on your assay and protein properties.

  • 10 mL of your buffer. Please bring your buffer recipe with you.
  • If applicable: different additives, detergents, buffer conditions, etc.

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