Walcutt_N_27November_2017

Graduate School of Oceanography - OCG 695
27 November, 3:30 PM, Coastal Institute Auditorium

Assessment of holographic microscopy for quantifying marine particle size and concentration

Achieving micron-scale measurements of marine particles and depth-resolved, ocean-basin coverage in sparse, highly variable oceanic environments persists as a methodological challenge. In situ holography may address this challenge by sampling 100x larger volumes than comparable objective lens-based systems, and is easily deployable on CTD-rosette, flow-through, and autonomous systems. Here, the quantitative capability of a digital in-line holographic microscope to evaluate size and concentration of particles ranging from 5 to 1000 microns in diameter is assessed. Over 500,000 particles are analyzed using a custom image processing pipeline, which allows a precise definition of the three-dimensional volume sampled. Recommendations for correcting the observed sizes and observed concentrations to the true sizes and concentrations are presented. The results of an inter-calibration with two other particle imaging systems, the FlowCam and the Imaging FlowCytobot, provide validation of the accuracy of these methods. Finally, calibrated size, concentration and aspect ratio statistics from CTD-mounted holographic microscope deployments are presented and some results that will guide the next steps of this project are discussed.

Noah Walcutt earned a B.E. in Mechanical Engineering from Vanderbilt University in 2008. He entered the M.S. program at GSO in Spring 2016. His major professor is Melissa Omand.

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* 1. EVALUATOR FIRST NAME

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* 2. EVALUATOR LAST NAME

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* 3. EVALUATOR ROLE

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* 4. What aspects of the presentation were successful? Why?

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* 5. What do you suggest to improve?

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* 6. ABSTRACT METHODS/RESULTS: Methods succinctly identified the methods used. Results are succinct and provide a specific explanation of what was discovered, accomplished, collected or produced.

EXCELLENT: Describes methodology in an organized, specific and concise manner. Describes what they have learned. Relates results to the research question. Results include quantitative data.

VERY GOOD: Discusses the methods or resources. States results only qualitatively.

GOOD: Discusses the methods or resources, but is lacking in specifics. Provides superficial explanation of results.

FAIR: Provides little or no discussion of the methods or resources used.
Provides no results or does not explain why results were not possible.

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* 7. ABSTRACT MOTIVATION/IMPACT: Succinctly describes and appropriately connects the subject and background to the purpose of the research . Provides a succinct interpretation of the results and evaluates what the results mean to the investigation and the field of study.

EXCELLENT: Describes how the investigation, whether completed or not, fits within a larger field or continuing investigation in a clear and concise manner.

VERY GOOD: Indicates main subject and purpose of the research. Provides an interpretation of results

GOOD: Indicates main subject and purpose of the research and indicates why research is important but is somewhat lacking in specifics. Provides an interpretation of results but fails to place results in the larger context of the field.

FAIR: Indicates main subject and purpose of the research but lacks organization, or lacks specific details or fails to make connections, or contains no introduction. Provides a conclusion that specificity or provides no conclusion.

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* 8. PRESENTATION ORGANIZATIONAL PATTERN: Specific introduction and conclusion, sequenced material within the body and transitions.

EXCELLENT: Organizational pattern is clearly and consistently observable and is skillful and makes the content of the presentation cohesive. Listener can follow the line of reasoning.

VERY GOOD: Organizational pattern is clearly and consistently observable

GOOD: Organizational pattern is intermittently observable. A few minor points may be confusing.

FAIR: Organizational pattern is not observable. Organization seems haphazard.

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* 9. PRESENTATION STYLE: Posture, gestures, eye contact and use of the voice. Speaker stands and moves with authority, looks more often at the audience than notes, uses the voice expressively, and uses few vocal fillers (‘um” “uh,” “like,” “you know,” etc.).

EXCELLENT: Delivery techniques (posture, gesture, eye contact, and vocal expressiveness) make the presentation compelling and the speaker appears polished and confident.

VERY GOOD: Delivery techniques (posture, gesture, eye contact, and vocal expressiveness) make the presentation interesting and speaker appears comfortable. Speaker uses pointer appropriately.

GOOD: Delivery techniques (posture, gesture, eye contact, and vocal expressiveness) make the presentation understandable and speaker appears tentative. Speaker reads from text rather than too the audience. Speaker does not use pointer to clarify material.

FAIR: Delivery techniques (posture, gesture, eye contact, and vocal expressiveness) detract from the understandability of the presentation and speaker appears uncomfortable

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* 10. PRESENTATION SUPPORTING MATERIAL: Slide contents, figures, table, supporting material support the presentation and facility the central message.

EXCELLENT: Font is large enough to be seen by all. Information is organized to maximize audience understanding. Details are minimized so that main points stand out. All figures/table clearly labeled.

VERY GOOD: Font size is appropriate. Appropriate information is included.

GOOD: Font size is appropriate. Appropriate information is included. Some material is not supported by visual aids. Colors may be used inappropriately. Layout is confusing.

FAIR: Font size is too small to be easily seen. Too much information is included, layout is crowded, not enough time is spent on each graphic to allow audience to follow along. Listeners become confused.

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* 11. PRESENTATION CENTRAL MESSAGE AND CONTENT: The main point/thesis/bottom line/take away of the presentation. A clear central message is easy to identify and compelling (broader impacts.)

EXCELLENT: Central message is precisely stated, appropriately repeated, memorable and strongly supported. Speaker provides accurate and complete explanation of key concepts and theories. Listeners gain insights. Information included is consistently accurate. Study is placed in large context.

VERY GOOD: Central message is clear and consistent and supported. For the most part, explanations of concepts and theories are accurate and complete. No significant errors.

GOOD: Central message is basically understandable but is not often repeated and is not memorable. Any significant errors are attributable to nervousness.

FAIR: Central message can be deduced but is not explicitly stated in the presentation. Explanations of concepts are inaccurate or incomplete. Listeners gain little from the presentation.

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* 12. PRESENTATION - ANSWER QUESTIONS: Speaker demonstrates understanding of material and answers the questions asked and is respectful.

EXCELLENT: Demonstrates deep understanding of topic.

VERY GOOD: Answers most questions with knowledge.

GOOD: Has some difficulty answering challenging questions.

FAIR: Does not understand the question or has difficulty answering questions.

Other (please specify)