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* 1. Name:

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* 2. Organization:

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* 3. Country:

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* 4. Select sequencing platforms that you have access to

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* 5. How many staff regularly perform sequencing in your laboratory?

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* 6. How many staff regularly perform bioinformatics analysis in your laboratory?

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* 7. Does your laboratory perform in-house bioinformatics analysis?

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* 8. What bioinformatics tools/programs/pipelines does your lab currently use?

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* 9. Which public repositories does your lab share data with?

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* 10. How are samples obtained for sequencing in your laboratory?

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* 11. What metadata is available for samples that are sequenced?

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* 12. Will sharing of sequencing data with PulseNet Africa members be possible?

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* 13. Does your lab participate in national surveillance for AMR of enteric bacteria?

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* 14. Does your lab participate in External Quality Control System for AST through Global Foodborne Infections Network (GFN)

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* 15. Does your lab upload data to the following programs? GLASS, WHO-NET

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* 16. Please describe your laboratory’s role in national enteric disease and AMR surveillance.

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* 17. Please provide your feasibility study proposal description. This should include the priority pathogen that will be sequenced, the one-health component of the project, any collaboration with other sites or institutions and desired public health impact (2 page limit, standard margins, font size Arial 10.5 or Times New Roman 12).

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* 18. Provide the source of isolates and % (i.e. 100% clinical, 50% clinical and 50% environmental)

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* 19. How will isolates be obtained?

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* 20. How many people will be participating in sequencing these isolates?

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* 21. How many people will plan on becoming PNI certified? (Note: Reagents provided will support certification strain sequencing for up to two staff)

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* 22. Do you foresee challenges in being able to complete sequencing of certification strains and feasibility bacterial enteric strains by August 30, 2025.

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* 23. If yes to previous question, please explain any challenges your laboratory might encounter in completing the sequencing of 100 isolates after receiving the sequencing reagents.

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