Reptile and Amphibian Journal Club - November 2023

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* 1. What is your first name?

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* 2. What is your last name?

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* 3. What is your email address?

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* 4. During the proliferation phase of wound healing, what cell type is responsible for producing angiogenic factors and vascular endothelial growth factor, resulting in dermal endothelial cells migrating into the ECM and forming granulation tissue?

Macrophages

Heterophils

Lymphocytes

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* 5. Why was manuka honey chosen over Silver sulfadiazine cream for dressing the wound after initial presentation?

Manuka honey produces local toxicity of fibroblasts and keratinocytes, with the possibility of bone marrow suppression and subsequent leukopenia when used in large wounds.

Manuka honey aids in autolytic debridement and has the added benefit of antibacterial properties, ability to reduce inflammation, and to reduce chronic inflammation while promoting macrophage and fibroblast migration into the wound.

Manuka honey regulates the repair by acquiring and managing several different cell types, which are coordinated during the different stages of healing.

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* 6. What is the extracellular matrix product made up of?

growth factors, interleukin, and tumor necrosis factors.

angiogenic factors and vascular endothelial growth factor.

fibrous components including collagens, elastins, laminins, fibrin, vitronectin, and fibrillin.

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* 7. What procedure was performed by the author that was likely unnecessary and may have prolonged the healing process?

Wound coverage with a non-adhesive polyurethane foam bandage material

Antimicrobial culture of the uncontaminated wounds

A single debridement was performed 7 days after the initial application of the ECM material.

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* 8. Which of the following are the phases of wound healing?

necrosis and tissue sloughing, growth factor production, and granulation tissue formation and endothelial cell migration

cellular infiltration and edema, debridement, and cytokine production, and granulation tissue formation

inflammation and contraction, proliferation, and maturation with subsequent remodeling