BIOTERRORISM REAGENT USE

* 2. How would you rate the difficulty in interpreting the following biochemical tests?

  Very easy Easy Somewhat easy Neither easy or difficult Somewhat difficult Difficult Very difficult N/A
Growth on Sheep Blood agar
Growth on Chocolate agar
Growth on MacConkey/EMB agar
Lactose fermentation on MacConkey agar
Growth on CNA/PEA
Growth on Martin Lewis/MTM
Growth on B. cepacia/Ashdown agar
Growth in Enrichment broth
Growth on BCYE agar
Growth on other cysteine-supplemental agar
Hemolysis
Gram stain appearance
Strength of staining
Colony morphology (Gram stain)
Organism size (Gram stain)
Chaining (Gram stain)
Spores present
Catalase
India Ink
Indole
Urease
Motility
Oxidase
Beta lactamase
Growth at 25-28°C
Growth at 42°C
Nitrate
XV requirement
Satellite
Colistin 
Polymixin B 
TSI/KIA
Arginine
Penicillin 
Amoxicillin-clavulanate

* 3. How would you rate the difficulty associated with the identification of the following organisms?

  Very easy Easy Somewhat easy Neither easy or difficult Somewhat difficult Difficult Very difficult N/A
Bacillus anthracis
Brucella spp.
Burkholderia mallei
Burkholderia pseudomallei
Francisella tualensis
Yersinia pestis

* 4. On a scale of 0-10 how would you rate your laboratory's overall bioterrorism preparedness with 1 being very unprepared and 10 being very prepared?

* 5. How would you compare your laboratory's overall bioterrorism preparedness today to what it was ten years ago?

* 6. How many years do you have performing microbiology biochemical tests?

* 7. How many years of experience do you have identifying select agents?

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