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2026 Flow Cytometry Course Post-Test
Unless otherwise stated, please answer each question to the best of your ability.
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1.
Name two major pre-analytical/sample handling variables that can affect the results of a flow cytometry assay.
(Required.)
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2.
What are the three major systems that make up a flow cytometer, and which system has the most frequent problems in daily use?
(Required.)
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3.
Define spillover spreading error and how it can be limited by good panel design.
(Required.)
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4.
Name four properties of adequate compensation/unmixing controls
(Required.)
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5.
Describe the purpose of single-stained controls and FMO controls for data interpretation and assay performance troubleshooting
(Required.)
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6.
Explain how instrument setup can be standardized across flow cytometers
(Required.)
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7.
List three reasons why certain events should be filtered (removed from analysis) prior to manual gating or clustering analysis.
(Required.)
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8.
What are the main factors that can decrease batch effects?
(Required.)
