OMB Control #0693-0033
Expiration Date: 06/30/2019

The purpose of this survey is to collect information, from the community, regarding potential reference grade tissue materials (e.g., tissues offered, what species, and how much tissue should we provide per vial). We want to make sure to provide materials that have value to the community and will be useful. 

In addition, using the developed reference grade materials, we also aim to conduct an interlaboratory comparison exercise for both lipids and metabolites. The core interlaboratory exercise will be to analyze, in triplicate, two prototype tissue-based reference grade materials (brain and liver) for lipids, metabolites, and/or both (whatever your laboratory routinely measures). Several questions below will let us know your interest in these activities, as well as provide feedback regarding how the exercises are designed. 

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* 1. PI name (none of the responses will be made public, this is just so we can follow-up with interested laboratories)

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* 2. For the healthy brain tissue reference grade material, what species do you find ideal?

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* 3. How much brain tissue (in mg) do you use (or would anticipate using) per single lipidomic/metabolomic analysis? We ask this question to help us determine the amount of tissue we should provide per vial.

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* 4. Would you find it useful to have a diseased brain tissue material as well?

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* 5. For healthy liver tissue reference grade material, what species do you find ideal?

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* 6. How much liver tissue (in mg) do you use (or would anticipate using) per single lipidomic/metabolomic analysis?

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* 7. Would you find it useful to have a diseased liver tissue as well? If so, which condition?

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* 8. Is there another tissue you think we should also offer?

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* 9. Do you have a preference as to how you would like the reference grade tissue materials offered?

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* 10. Do you find any value in NIST offering pre-extracted tissue lipid/metabolite materials as well?

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* 11. Are you interested in participating in an interlaboratory exercise focused on evaluating these tissue materials (quantitatively)? Note that while your answer is not binding, by selecting yes, you agree to receive updates regarding the initiation of this exercise.

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* 12. If yes, what will your laboratory measure?

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* 13. How long would you need to make triplicate measurements of these tissue reference materials? Note that we are anticipating/projecting to have the materials ready to distribute by the beginning of 2018.

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* 14. Would you also be interested in participating in a longitudinal study using these tissue materials? To evaluate the effectiveness of these reference grade materials over time, we are asking for a select number of laboratories to be willing to make triplicate measurements of these materials once per year for 3 years.

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* 15. Other activities are planned for the upcoming year and beyond. Please let us know your interest, if any.  If interested, we will put you on our list and you will receive updates on the progress of these potential activities. Select those that you are interested in (more than one option may apply)

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* 16. For the laboratories that perform metabolomics, would you be interested in quantitatively analyzing the metabolome of SRM 1950 (in triplicate)? Or if you have already analyzed SRM 1950 in this manner, would you be willing to provide us your data (and/or concentration values) and methods? The intention of this request, is to calculate consensus means with associated uncertainties for the metabolome in SRM 1950, similar to the lipidomic quantitative analysis of this material. The community-derived consensus means (using submitted data) will be publicly accessible and could serve as benchmarks to promote harmonization within the community.

This collection of information contains Paperwork Reduction Act (PRA) requirements approved by the Office of Management and Budget (OMB). Notwithstanding any other provisions of the law, no person is required to respond to, nor shall any person be subject to a penalty for failure to comply with, a collection of information subject to the requirements of the PRA unless that collection of information displays a currently valid OMB control number. Public reporting burden for this collection is estimated to be 7 minutes total, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed and completing and reviewing the collection of information. Send comments regarding this burden estimate or any aspect of this collection of information, including suggestions for reducing this burden, to the National Institute of Standards and Technology, Attn: John A. Bowden (john.bowden@nist.gov).

OMB Control No. 0693-0033
Expiration Date: 06/30/2019

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