Thank you for your interest in Nikon Instruments. Please fill out this survey so that we can customize a confocal microscope system for you.

What position do you hold at TAMU?

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* 1. What position do you hold at TAMU?

Do your samples require an UPRIGHT or INVERTED Microscope

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* 2. Do your samples require an UPRIGHT or INVERTED Microscope

What objective magnifications do you require?

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* 3. What objective magnifications do you require?

What sort of samples will you need to image?

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* 4. What sort of samples will you need to image?

Are your samples mounted in PLASTIC bottom well plates/dishes?

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* 5. Are your samples mounted in PLASTIC bottom well plates/dishes?

Do you require contrast techniques such as Phase Contrast or DIC (Differential Interference Contrast)?

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* 6. Do you require contrast techniques such as Phase Contrast or DIC (Differential Interference Contrast)?

If you answered "Yes" to Question 6, then at what magnifications are Phase Contrast or DIC needed?

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* 7. If you answered "Yes" to Question 6, then at what magnifications are Phase Contrast or DIC needed?

Please select how your samples will be mounted for imaging? Please check all that apply.

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* 8. Please select how your samples will be mounted for imaging? Please check all that apply.

What is the level of resolution that you require for your experiments? Please check all that apply.

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* 9. What is the level of resolution that you require for your experiments? Please check all that apply.

For those imaging tissues, how deep into the sample do you need to image?

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* 10. For those imaging tissues, how deep into the sample do you need to image?

How dynamic are the events that you will want to image? What is the frame rate per second (FPS) that you require?

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* 11. How dynamic are the events that you will want to image? What is the frame rate per second (FPS) that you require?

For live-cell experiments, please select below all that would apply:

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* 12. For live-cell experiments, please select below all that would apply:

For live-cell experiments, please select the typical total length/s of time per experiment. Please describe what you would like to image live.

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* 13. For live-cell experiments, please select the typical total length/s of time per experiment. Please describe what you would like to image live.

If you are interested in Multiphoton Confocal capacity, then please describe the types of experiments you plan to image.

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* 14. If you are interested in Multiphoton Confocal capacity, then please describe the types of experiments you plan to image.

Select fluorophores that you will image

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* 15. Select fluorophores that you will image

How important is High Speed Confocal Imaging to your research? Speeds with a Resonant Scanner: 30 fps in full frame, 420 fps in band scan, 15,600 fps in line scan mode

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* 16. How important is High Speed Confocal Imaging to your research? Speeds with a Resonant Scanner: 30 fps in full frame, 420 fps in band scan, 15,600 fps in line scan mode

How important is it to have a Spectral Detector for your research? It is used to separate closely emitting fluors such as GFP and YFP, separate Background Fluorescence, and spectral FRET.

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* 17. How important is it to have a Spectral Detector for your research? It is used to separate closely emitting fluors such as GFP and YFP, separate Background Fluorescence, and spectral FRET.

How important is it to have the capacity to do photoactivation, photobleaching, or photoconversion for your research?

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* 18. How important is it to have the capacity to do photoactivation, photobleaching, or photoconversion for your research?

How important is it to have the capacity to do photoactivation, photobleaching, or photoconversion while imaging SIMULTANEOUSLY for your research? e.g., Imaging the rapid diffusion of a subset of proteins that you've just photoconverted from Green to Red

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* 19. How important is it to have the capacity to do photoactivation, photobleaching, or photoconversion while imaging SIMULTANEOUSLY for your research? e.g., Imaging the rapid diffusion of a subset of proteins that you've just photoconverted from Green to Red

How important is the capacity to incorporate other microscopy techniques such as super-resolution (SIM, STED, STORM, PALM), FLIM (fluorescence lifetime imaging microscopy), or FCS (fluorescence correlation spectroscopy) into your research?

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* 20. How important is the capacity to incorporate other microscopy techniques such as super-resolution (SIM, STED, STORM, PALM), FLIM (fluorescence lifetime imaging microscopy), or FCS (fluorescence correlation spectroscopy) into your research?

How important is the ability to do High-Content Screening/Analysis for your research needs? i.e. Scanning Well-Plates and performing analysis on image datasets

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* 21. How important is the ability to do High-Content Screening/Analysis for your research needs? i.e. Scanning Well-Plates and performing analysis on image datasets

Please rank the following according to their order of importance with regards to a new microscope system:

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* 22. Please rank the following according to their order of importance with regards to a new microscope system:

Are there any other questions, features, or capacities that you require that were not covered by this survey?

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* 23. Are there any other questions, features, or capacities that you require that were not covered by this survey?

Please share your name and email address so that we can contact you about your specific experimental needs as we finalize the features for your custom confocal microscope system.

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* 24. Please share your name and email address so that we can contact you about your specific experimental needs as we finalize the features for your custom confocal microscope system.

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