Thank you for your interest in Nikon Instruments. Please fill out this survey so that we can customize a confocal microscope system for you.

* 1. What position do you hold at TAMU?

* 2. Do your samples require an UPRIGHT or INVERTED Microscope

* 3. What objective magnifications do you require?

* 4. What sort of samples will you need to image?

* 5. Are your samples mounted in PLASTIC bottom well plates/dishes?

* 6. Do you require contrast techniques such as Phase Contrast or DIC (Differential Interference Contrast)?

* 7. If you answered "Yes" to Question 6, then at what magnifications are Phase Contrast or DIC needed?

* 8. Please select how your samples will be mounted for imaging? Please check all that apply.

* 9. What is the level of resolution that you require for your experiments? Please check all that apply.

* 10. For those imaging tissues, how deep into the sample do you need to image?

* 11. How dynamic are the events that you will want to image? What is the frame rate per second (FPS) that you require?

* 12. For live-cell experiments, please select below all that would apply:

* 13. For live-cell experiments, please select the typical total length/s of time per experiment. Please describe what you would like to image live.

* 14. If you are interested in Multiphoton Confocal capacity, then please describe the types of experiments you plan to image.

* 15. Select fluorophores that you will image

* 16. How important is High Speed Confocal Imaging to your research? Speeds with a Resonant Scanner: 30 fps in full frame, 420 fps in band scan, 15,600 fps in line scan mode

* 17. How important is it to have a Spectral Detector for your research? It is used to separate closely emitting fluors such as GFP and YFP, separate Background Fluorescence, and spectral FRET.

* 18. How important is it to have the capacity to do photoactivation, photobleaching, or photoconversion for your research?

* 19. How important is it to have the capacity to do photoactivation, photobleaching, or photoconversion while imaging SIMULTANEOUSLY for your research? e.g., Imaging the rapid diffusion of a subset of proteins that you've just photoconverted from Green to Red

* 20. How important is the capacity to incorporate other microscopy techniques such as super-resolution (SIM, STED, STORM, PALM), FLIM (fluorescence lifetime imaging microscopy), or FCS (fluorescence correlation spectroscopy) into your research?

* 21. How important is the ability to do High-Content Screening/Analysis for your research needs? i.e. Scanning Well-Plates and performing analysis on image datasets

* 22. Please rank the following according to their order of importance with regards to a new microscope system:

* 23. Are there any other questions, features, or capacities that you require that were not covered by this survey?

* 24. Please share your name and email address so that we can contact you about your specific experimental needs as we finalize the features for your custom confocal microscope system.

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