Introduction to Basic SFC Quiz Question Title * 1. What two requirements are needed to make a supercritical fluid? (Select two) Temperature Flow Pressure Vacuum Density Question Title * 2. What values are the critical point for carbon dioxide? 31.1 °C, 73.8 bar 32.2 °C, 48.7 bar 239.5 °C, 80.8 bar 374 °C, 221 bar Question Title * 3. Who noticed a convergence between high pressure gas chromatography and liquid chromatography? Ernst Klesper James Locklove J.C. Giddings Terry Berger Question Title * 4. Which pump is required to prevent precipitation into the MS? CO2 pump Modifier delivery pump Make up pump Question Title * 5. Why should a column be flushed in 100% methanol if used on both a HPLC and SFC instrument? Column is most stable stored in methanol To improve separation efficiency To prevent contamination To flush the water out of the column before introducing CO2 Question Title * 6. What polarity range of compounds can SFC cover using CO2/MeOH as the mobile phase? 10 to -10 10 to -1 10 to 5 0 to -5 Question Title * 7. Is the cosolvent more polar than CO2? Yes No Question Title * 8. What advantage does SFC offer over HPLC? Limited application range Lower equipment cost Faster separation Information on the conductivity of the analytes Question Title * 9. Can water be used in the organic modifier in SFC? Not at all Yes but typically 1-5% of the modifier Yes, it can be freely used C18 - nitrobenzene, trinitrobenzenePhenyl - trinitrobenzene, nitrobenzene Question Title * 10. What advantage does the LotusStream GLS offer over traditional approaches? (Select all that apply) Greater recovery Low carryover Available for small analytical scale through to larger prep scale Minimise sample dispersion Question Title * 11. To be entered into the prize draw, please fill in your details below Name Company ZIP/Postal Code Country Email Address Done
