Introduction to Basic SFC Quiz

Question Title

1. What two requirements are needed to make a supercritical fluid? (Select two) (Required.)

Temperature

Flow

Pressure

Vacuum

Density

Question Title

2. What values are the critical point for carbon dioxide? (Required.)

31.1 °C, 73.8 bar

32.2 °C, 48.7 bar

239.5 °C, 80.8 bar

374 °C, 221 bar

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3. Who noticed a convergence between high pressure gas chromatography and liquid chromatography? (Required.)

Ernst Klesper

James Locklove

J.C. Giddings

Terry Berger

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4. Which pump is required to prevent precipitation into the MS? (Required.)

CO2 pump

Modifier delivery pump

Make up pump

Question Title

5. Why should a column be flushed in 100% methanol if used on both a HPLC and SFC instrument? (Required.)

Column is most stable stored in methanol

To improve separation efficiency

To prevent contamination

To flush the water out of the column before introducing CO2

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6. What polarity range of compounds can SFC cover using CO2/MeOH as the mobile phase? (Required.)

10 to -10

10 to -1

10 to 5

0 to -5

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7. Is the cosolvent more polar than CO2? (Required.)

Yes

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8. What advantage does SFC offer over HPLC? (Required.)

Limited application range

Lower equipment cost

Faster separation

Information on the conductivity of the analytes

Question Title

9. Can water be used in the organic modifier in SFC? (Required.)

Not at all

Yes but typically 1-5% of the modifier

Yes, it can be freely used

C18 - nitrobenzene, trinitrobenzene
Phenyl - trinitrobenzene, nitrobenzene

Question Title

10. What advantage does the LotusStream GLS offer over traditional approaches? (Select all that apply) (Required.)

Greater recovery

Low carryover

Available for small analytical scale through to larger prep scale

Minimise sample dispersion

Introduction to Basic SFC Quiz

Question Title

1. What two requirements are needed to make a supercritical fluid? (Select two) (Required.)

Question Title

2. What values are the critical point for carbon dioxide? (Required.)

Question Title

3. Who noticed a convergence between high pressure gas chromatography and liquid chromatography? (Required.)

Question Title

4. Which pump is required to prevent precipitation into the MS? (Required.)

Question Title

5. Why should a column be flushed in 100% methanol if used on both a HPLC and SFC instrument? (Required.)

Question Title

6. What polarity range of compounds can SFC cover using CO2/MeOH as the mobile phase? (Required.)

Question Title

7. Is the cosolvent more polar than CO2? (Required.)

Question Title

8. What advantage does SFC offer over HPLC? (Required.)

Question Title

9. Can water be used in the organic modifier in SFC? (Required.)

Question Title

10. What advantage does the LotusStream GLS offer over traditional approaches? (Select all that apply) (Required.)

Question Title

11. Full Name (Required.)

Question Title

12. Email Address (Required.)

Question Title

13. Company / Institution (Required.)

Question Title

14. Post Code (Required.)

Question Title

15. Telephone Number

Introduction to Basic SFC Quiz

Question Title

* 1. What two requirements are needed to make a supercritical fluid? (Select two) (Required.)

Question Title

* 2. What values are the critical point for carbon dioxide? (Required.)

Question Title

* 3. Who noticed a convergence between high pressure gas chromatography and liquid chromatography? (Required.)

Question Title

* 4. Which pump is required to prevent precipitation into the MS? (Required.)

Question Title

* 5. Why should a column be flushed in 100% methanol if used on both a HPLC and SFC instrument? (Required.)

Question Title

* 6. What polarity range of compounds can SFC cover using CO2/MeOH as the mobile phase? (Required.)

Question Title

* 7. Is the cosolvent more polar than CO2? (Required.)

Question Title

* 8. What advantage does SFC offer over HPLC? (Required.)

Question Title

* 9. Can water be used in the organic modifier in SFC? (Required.)

Question Title

* 10. What advantage does the LotusStream GLS offer over traditional approaches? (Select all that apply) (Required.)

Question Title

* 11. Full Name (Required.)

Question Title

* 12. Email Address (Required.)

Question Title

* 13. Company / Institution (Required.)

Question Title

* 14. Post Code (Required.)

Question Title

* 15. Telephone Number

1. What two requirements are needed to make a supercritical fluid? (Select two) (Required.)

2. What values are the critical point for carbon dioxide? (Required.)

3. Who noticed a convergence between high pressure gas chromatography and liquid chromatography? (Required.)

4. Which pump is required to prevent precipitation into the MS? (Required.)

5. Why should a column be flushed in 100% methanol if used on both a HPLC and SFC instrument? (Required.)

6. What polarity range of compounds can SFC cover using CO2/MeOH as the mobile phase? (Required.)

7. Is the cosolvent more polar than CO2? (Required.)

8. What advantage does SFC offer over HPLC? (Required.)

9. Can water be used in the organic modifier in SFC? (Required.)

10. What advantage does the LotusStream GLS offer over traditional approaches? (Select all that apply) (Required.)

11. Full Name (Required.)

12. Email Address (Required.)

13. Company / Institution (Required.)

14. Post Code (Required.)

15. Telephone Number