Please provide your details below before starting the assessment:

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1. Name and Surname: (Required.)

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2. Staff / Student Number (if you are not a UFS staff member or student, you can enter "NA"): (Required.)

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3. Department Name (if you are not with a department at UFS, you can enter "NA"): (Required.)

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4. Email Address: (Required.)

Section 1: Module 1, Introduction to Biosafety and Biosecurity, Part 1 and 2
Core Concepts

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5. What is the main goal of biosafety in a laboratory setting?

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6. Which of the following best describes biosecurity?

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7. Bio-risk management includes which of the following elements?

Practices and Equipment

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8. Which of the following is NOT considered a standard microbiological practice?

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9. The best place to work with infectious biological materials:

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10. What is the primary function of a biosafety cabinet?

Biosafety Levels

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11. Which biosafety level (BSL) is required for working with agents not known to cause disease?

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12. Which biosafety level is needed for working with Ebola virus?

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13. Which of the following is a feature of BSL-3 laboratories?

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14. What is a typical requirement before entering a BSL-4 lab?

Risk Assessment and Management

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15. The foundation for determining appropriate biosafety levels in a laboratory:

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16. What is the main purpose of a risk assessment in bio-risk management?

Section 2: Module 2, Concepts of Risk Management, Part 1 and 2

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17. Which of the following is an example of a biohazard?

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18. What is the primary goal of biosafety?

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19. Who would be involved in a risk assessment?

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20. Which is the most effective method for biohazard control?

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21. The ‘five P's’ in risk assessment include all EXCEPT:

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22. True or false: PPE should always be considered the first line of defence against biohazards?

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23. True or false: Decontamination refers to the removal or inactivation of biological agents?

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24. True or false: Risk assessments never need to be updated after they are first completed?

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25. True or false: Substitution involves replacing a hazardous agent with a less hazardous one if elimination is not possible?

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26. True or false: Training and written procedures are examples of administrative controls?

Section 3: Module 3, Biosafety Laboratory (BSL-1 and BSL-2), Part 1 and 2

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27. Which legislation provides regulations related to laboratory safety and requires employers in South Africa to ensure the health and safety of persons at work?

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28. Who is responsible for laboratory safety within the laboratory?

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29. What items are considered biosafety personal protective equipment (PPE)? (Select three that apply) (Required.)

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30. What additional PPE may be required for BSL-2 laboratories compared to BSL-1?

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31. Which of the following is NOT a category of biohazard waste?

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32. What is the recommended method for decontaminating liquid biohazard waste?

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33. Which chemical is NOT commonly used for decontamination in laboratories?

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34. What is the first step in responding to a biological spill in the laboratory?

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35. Which laboratory sign indicates a mandatory action such as wearing PPE?

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36. Which of the following best describes the difference between BSL-1 and BSL-2 laboratories?

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