Unless otherwise stated, please answer each question to the best of your ability.

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1. Name two major pre-analytical/sample handling variables that can affect the results of a flow cytometry assay. (Required.)

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2. What are the three major systems that make up a flow cytometer, and which system has the most frequent problems in daily use? (Required.)

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3. Define spillover spreading error and how it can be limited by good panel design. (Required.)

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4. Name four properties of adequate compensation/unmixing controls (Required.)

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5. Describe the purpose of single-stained controls and FMO controls for data interpretation and assay performance troubleshooting (Required.)

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6. Explain how instrument setup can be standardized across flow cytometers (Required.)

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7. List three reasons why certain events should be filtered (removed from analysis) prior to manual gating or clustering analysis. (Required.)

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8. What are the main factors that can decrease batch effects? (Required.)

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