CLSI M100 is a critical resource for antimicrobial susceptibility testing (AST) and clinical decision‑making. This survey is intended to better understand how laboratories access and use CLSI breakpoints, how breakpoint changes are interpreted and implemented, and where challenges may arise. We appreciate your time and feedback.

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* 1. Let's start. What format do you use to access CLSI breakpoints?
Select all that apply.

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* 2. Do you purchase a new edition when CLSI M100 is updated every year?

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* 3. Do you use other CLSI Breakpoint guidance in your laboratory?

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* 4. Who in your organization is primarily responsible for interpreting CLSI M100 guidance?

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* 5. When you receive a new edition of CLSI M100, what is the first thing to happen?

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* 6. Where do you experience the greatest friction in using CLSI M100 today?

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* 7. Do you use breakpoints from other organizations in your laboratory?

IMPLEMENTATION

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* 8. How do you typically determine whether breakpoint changes affect your laboratory?

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* 9. How are new or updated breakpoints entered into your systems?

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* 10. How are CLSI annotations or footnotes handled?

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* 11. Which types of conditional guidance are hardest to implement technically?

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* 12. How do you manage multiple breakpoint versions in your environment?

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* 13. How does your laboratory document which breakpoints are in use?

BREAKPOINT INTERPRETATION

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* 14. Which steps are most resource-intensive?

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* 15. How confident are you that your laboratory consistently applies CLSI breakpoints as intended?

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* 16. What makes annotations or footnotes most challenging to implement?

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* 17. What factors most commonly delay adoption of updated CLSI breakpoints?

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* 18. How are breakpoint implementation errors typically discovered?

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* 19. Which CLSI‑provided tools would most improve breakpoint implementation accuracy?

WRAP-UP
Last few questions to help us analyze responses!

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* 20. What AI tools does your organization currently use or allow?

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* 21. How would you characterize your overall sentiment toward AI right now?

Negative/overwhelmed Positive/excited
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i We adjusted the number you entered based on the slider’s scale.

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* 22. What is your role?

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* 23. Which best describes your organization?

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* 24. Where is your work located?

T