Introduction to ABC Atlas

Thank you for attending this webinar about Introduction to ABC Atlas with the Allen Institute. Your feedback is extremely valuable in helping us better develop resources for you, and we appreciate your responses to this survey.

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* 1. How much do you understand transcriptomics?

Beginner: Only what was taught in my classes or I read in papers

Intermediate: I know about it but I do not use it in my research

Advance: I use it in my research but not the primary focus

Expert: I use MERFISH/10x/SMARTseq/similar techniques almost daily

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* 2. What dataset in ABC Atlas (as of May 2024) interests you?

	Very interested	Somewhat interested	Neutral	Barely interested	Not interested at all
Whole Mouse Brain – 10x (single cell)	Whole Mouse Brain – 10x (single cell) Very interested	Whole Mouse Brain – 10x (single cell) Somewhat interested	Whole Mouse Brain – 10x (single cell) Neutral	Whole Mouse Brain – 10x (single cell) Barely interested	Whole Mouse Brain – 10x (single cell) Not interested at all
Whole Mouse Brain - MERFISH (Zeng data: spatial, coronal, anatomical boundaries)	Whole Mouse Brain - MERFISH (Zeng data: spatial, coronal, anatomical boundaries) Very interested	Whole Mouse Brain - MERFISH (Zeng data: spatial, coronal, anatomical boundaries) Somewhat interested	Whole Mouse Brain - MERFISH (Zeng data: spatial, coronal, anatomical boundaries) Neutral	Whole Mouse Brain - MERFISH (Zeng data: spatial, coronal, anatomical boundaries) Barely interested	Whole Mouse Brain - MERFISH (Zeng data: spatial, coronal, anatomical boundaries) Not interested at all
Whole Mouse Brain – MERFISH (Zhuang data: spatial, sagittal and coronal)	Whole Mouse Brain – MERFISH (Zhuang data: spatial, sagittal and coronal) Very interested	Whole Mouse Brain – MERFISH (Zhuang data: spatial, sagittal and coronal) Somewhat interested	Whole Mouse Brain – MERFISH (Zhuang data: spatial, sagittal and coronal) Neutral	Whole Mouse Brain – MERFISH (Zhuang data: spatial, sagittal and coronal) Barely interested	Whole Mouse Brain – MERFISH (Zhuang data: spatial, sagittal and coronal) Not interested at all
Whole Human Brain – 10x Neurons (single nucleus)	Whole Human Brain – 10x Neurons (single nucleus) Very interested	Whole Human Brain – 10x Neurons (single nucleus) Somewhat interested	Whole Human Brain – 10x Neurons (single nucleus) Neutral	Whole Human Brain – 10x Neurons (single nucleus) Barely interested	Whole Human Brain – 10x Neurons (single nucleus) Not interested at all
Whole Human Brain – 10x Non-neurons (single nucleus)	Whole Human Brain – 10x Non-neurons (single nucleus) Very interested	Whole Human Brain – 10x Non-neurons (single nucleus) Somewhat interested	Whole Human Brain – 10x Non-neurons (single nucleus) Neutral	Whole Human Brain – 10x Non-neurons (single nucleus) Barely interested	Whole Human Brain – 10x Non-neurons (single nucleus) Not interested at all
Human Alzheimer’s – 10x MTG and DLPFC (single nucleus)	Human Alzheimer’s – 10x MTG and DLPFC (single nucleus) Very interested	Human Alzheimer’s – 10x MTG and DLPFC (single nucleus) Somewhat interested	Human Alzheimer’s – 10x MTG and DLPFC (single nucleus) Neutral	Human Alzheimer’s – 10x MTG and DLPFC (single nucleus) Barely interested	Human Alzheimer’s – 10x MTG and DLPFC (single nucleus) Not interested at all
Human Alzheimer’s – MERFISH MTG (spatial)	Human Alzheimer’s – MERFISH MTG (spatial) Very interested	Human Alzheimer’s – MERFISH MTG (spatial) Somewhat interested	Human Alzheimer’s – MERFISH MTG (spatial) Neutral	Human Alzheimer’s – MERFISH MTG (spatial) Barely interested	Human Alzheimer’s – MERFISH MTG (spatial) Not interested at all

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* 3. What are some ways you could see yourself using ABC Atlas?

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* 4. What resources would you like Allen Institute to create to help understand ABC Atlas research? Select all that apply

In-depth transcriptomics introduction (webinar)

In-depth transcriptomics introduction (written guide)

In-depth spatial transcriptomics introduction (webinar)

Dedicated user guides for each dataset

Single-cell vs single-nucleus RNAseq question guide

More use case examples

Virtual office hours; opportunity to ask Allen scientists about using ABC Atlas

Other (please specify)

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* 5. What features would you like to see ABC Atlas have?

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* 6. Job Title/Position:

Current undergraduate student

Research technician/associate

Current graduate student (Masters or PhD)

Postdoc

Staff scientist (PhD or equivalent)

Non-tenured PI or equivalent

Tenured PI or equivalent

Science adjacent position (engineer, scicomm, etc)

Other

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* 7. Do you plan on using ABC Atlas after this webinar?

Yes

Unsure

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* 8. What is your affiliation/institution

Thank you for attending our webinar and answering our feedback. If you have any additional comments or concerns, please email education@alleninstitute.org.

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* 9. Have you used ABC Atlas before?

Yes