[7Q] Quiz SARS-CoV-2 Whole genome sequencing workflow Question Title * 1. Please enter your name, country and email address below so we can register who performed the exercise and submitted their answers (optional). Note! This information will be saved to keep a record of candidates for potential future training courses (virtual or in-person). Name Country Email Address Question Title * 2. Arrange the following in the correct workflow order a. QC with bioanalyzer b. Quantification c. Amplicon generation (enrichment) d. Library prep c, a, b, d c, b, d, a a, b, c, d None of the above Question Title * 3. True or false: The Agilent bioanalyzer employs laser induced fluorescence True False Question Title * 4. True or false: During the amplicon generation step (enrichment), the most important section of the coronavirus genome is amplified and taken into library prep. True False Question Title * 5. Which of the following applies to Qubit Quantification? Double stranded cDNA can be quantified exclusively It makes use of UV and UV absorbance in its operation dsDNA can be quantified exclusively, but not double stranded cDNA The higher the fluorescence emitted, the lower the concentration recorded Question Title * 6. With Illumina Nextera library prep, the function of the transposome is to Denature any proteins that might have made it into the extracts Fragment the DNA and add adapter sequences Add indexes to the fragments Decrease the activation energy for other fragmenting enzymes to act Question Title * 7. Which of the following steps in Illumina Nextera library prep is attributed with fragment size selection? QC Step Bead clean up step PCR Step Amplicon generation step Question Title * 8. True or false: The Agilent Bioanalyzer cannot quantify cDNA, but can generate average fragment sizes. True False Question Title * 9. During the bead clean-up process, the magnetic stand Attracts the beads to the side of the tubes/ wells, aiding in the clean-up step Automatically size select the fragments of interest Attracts the DNA fragments to the side of the tubes/wells Enhances enzymatic action in the tubes/wells Question Title * 10. What is the function of the indexes during library prep? It helps with the clean-up step It gives identity to each sample It is needed for the bioanalyzer to function with regards to quantification It is needed to stop the fragmentation step during library prep Question Title * 11. True or false: Nanodrop is as good as the Qubit with regards to quantification for sequencing True False Question Title * 12. During the amplicon PCR step, why is it important not to mix the two primer pools? To prevent the primers from the different pools from pairing with each other To make the reaction go faster To lower the annealing temperature of the reaction Page1 / 4 25% of survey complete. Next