ONT 16S Analysis Survey

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* 1. Contact Information

Name

Company

Title

Email Address

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* 2. How often do you use Oxford Nanopore long read sequencing technology for 16S amplicon analysis?

Every month

Every few months

Once per year or less

I own an instrument but have not used it

Additional Comments:

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* 3. Which Oxford Nanopore instrument are you currently running 16S analysis on? (check all that apply)

MinION

GridION

PromethION

Comments:

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* 4. How are you currently preparing your 16S amplicons for your Oxford Nanopore experiments?

I currently the Oxford Nanopore 16S amplicon prep kit

I use my own set of 16S primers

I have used both

I use another commercial kit

Please provide additional information on kits used:

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* 5. What sample types are you currently testing using 16S analysis on Oxford Nanopore? (check all that apply)

Fecal samples

Gut contents

Skin samples

Wastewater samples

Other types of water samples

Vaginal samples

Nasal samples

Surface swabs

Soil samples

Milk samples

Food samples

Tissue samples

Plant samples

Please comment on other sample types and issues you have had with specific sample types:

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* 6. Please comment on how you are currently analyzing your 16S data output from your Oxford Nanopore runs, any gaps/bottleneck you encounter and the level of taxonomic resolution you can currently achieve?

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* 7. Do you see a need for single sample analysis as well as sample multiplexing?

Yes

How many samples would you ideally like to multiplex on a single flow cell?

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* 8. What level of taxonomic resolution do you desire to achieve using the Oxford Nanopore long read sequencing technology?

Species level is acceptable

Strain level is needed

Will be dependent on experiment goals

Below species level is acceptable

What level of taxonomic resolution are you currently getting using your ONT 16S assay:

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* 9. What applications do you focus on now and do you see in the future that are a good fit for 16S amplicon analysis on Oxford Nanopore?

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* 10. What would be an acceptable price point for a strain level resolution long read amplicon kit for that also included microbial lysis, DNA purification, PCR as well as analysis software?

$100-75 per sample

$75-50 per sample

$50-25 per sample

less than $25 per sample

I would not pay for a complete solution

Please comment as to the reasons to your answer: