ABRF PSRG 2012 Survey

1. Please enter your Laboratory Identification Number (the anonymous identifier for your lab that you received via email):

Please enter your Laboratory Identification Number (the anonymous identifier for your lab that you received via email):

2. What types of analyses did you perform on the sample? Check all that apply.

What types of analyses did you perform on the sample? Check all that apply. Top -down mass spectrometry analysis

Bottom-up mass spectrometry analysis

Edman degradation

In Source Decay

T3 Sequencig

ECD/ETD

Tandem MS

Intact MW Measurement

Electroelution

Biochemical Labeling or Capture

Other (please specify)

3. What methods, if any, did you use to separate and purify the sample?

What methods, if any, did you use to separate and purify the sample? Used sample as provided

SDS_PAGE

HPLC

Other (please specify)

4. Which samples did you attempt for analysis? Check all that apply

Which samples did you attempt for analysis? Check all that apply Bovine serum albumin (BSA)

Protein A

Endostatin (Collagen fragment)

5. Was there sufficient material in order to confidently call the N-terminus of the protein?

	Yes	No
BSA	*Was there sufficient material in order to confidently call the N-terminus of the protein? BSA Yes	BSA No
Protein A	Protein A Yes	Protein A No
Endostatin (Collagen fragment)	Endostatin (Collagen fragment) Yes	Endostatin (Collagen fragment) No

6. If yes to #5, how much material did you use in order to confidently call the N-terminus of the protein? Please specify units.

If yes to #5, how much material did you use in order to confidently call the N-terminus of the protein? Please specify units. BSA
Protein A
Endostatin (Collagen fragment)

7. If no to #5, how much more material would you need in order to confidently call the N-terminus of the protein? Please specify units.

If no to #5, how much more material would you need in order to confidently call the N-terminus of the protein? Please specify units. BSA
Protein A
Endostatin (Collagen fragment)

8. Please list the amino acid sequence (in single letter code) that you were able to CONFIDENTLY call for the N-terminus of the protein using:

Please list the amino acid sequence (in single letter code) that you were able to CONFIDENTLY call for the N-terminus of the protein using: BSA
Protein A
Endostatin (Collagen fragment)

If Edman degradation only used for sample analysis skip to page 3 or question # 18.

ABRF PSRG 2012 Survey
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General Questions

1. Please enter your Laboratory Identification Number (the anonymous identifier for your lab that you received via email):

2. What types of analyses did you perform on the sample? Check all that apply.

3. What methods, if any, did you use to separate and purify the sample?

4. Which samples did you attempt for analysis? Check all that apply

5. Was there sufficient material in order to confidently call the N-terminus of the protein?

6. If yes to #5, how much material did you use in order to confidently call the N-terminus of the protein? Please specify units.

7. If no to #5, how much more material would you need in order to confidently call the N-terminus of the protein? Please specify units.

8. Please list the amino acid sequence (in single letter code) that you were able to CONFIDENTLY call for the N-terminus of the protein using:

ABRF PSRG 2012 SurveyExit this survey

General Questions

1. Please enter your Laboratory Identification Number (the anonymous identifier for your lab that you received via email):

2. What types of analyses did you perform on the sample? Check all that apply.

3. What methods, if any, did you use to separate and purify the sample?

4. Which samples did you attempt for analysis? Check all that apply

5. Was there sufficient material in order to confidently call the N-terminus of the protein?

6. If yes to #5, how much material did you use in order to confidently call the N-terminus of the protein? Please specify units.

7. If no to #5, how much more material would you need in order to confidently call the N-terminus of the protein? Please specify units.

8. Please list the amino acid sequence (in single letter code) that you were able to CONFIDENTLY call for the N-terminus of the protein using:

ABRF PSRG 2012 Survey
Exit this survey