General Information:
Location:
Hyatt Regency Cambridge
575 Memorial Drive
Cambridge, MA 02139
Seminar Times:
10:00 AM to 12:00 PM and 1:30 PM to 3:30 PM
Refreshments will be provided.
Please use the "next" button to move through to the registration form.
Abstracts:
I. What Differentiates Multi-Modal Cation Exchange Resins from Traditional Cation Exchange Resins?
Mixed mode chromatography has proven to be a unique separation strategy in the purification process development toolbox. In recent years there have been several multimodal cation exchanger resins designed and commercially launched including CaptoMMC from GE Healthcare, Toyopearl MX-Trp-650M from Tosoh Bioscience and NuviaTM cPrimeTM from Biorad. How do these multimodal cation exchange resins differ from traditional cation exchange resins in their separation mechanism and application performance? Cation exchange chromatography, especially methods using the high-capacity new generation cation exchange resins, has a successful track record in biopharmaceutical manufacturing processes. This raises the question, where do multimodal cation exchangers fit? In this presentation, we present new methodology for multimodal cation exchange chromatographic process development. Through some case studies we address different throughput benefits of traditional vs multimodal cation exchange resins, and the unique separation resolution advantages offered by multimodal cation exchange resins during the manufacturing process development for monoclonal antibody products.
Presented by: Jie Chen
Director of Protein Sciences, Dyax Corp.
II. The Use of Dual Salts to Improve Capacity and Selectivity in HIC and Mixed-Mode Resins as a Logical Extension to HIC Applications.
Hydrophobic interaction chromatography (HIC) is a unique mode that offers several advantages over ion exchange and size exclusion chromatography. HIC is often used to remove aggregates, host cell proteins and leached Protein A. Recent advances have shown that using a dual salt combination (mixing kosmotropic and chaotropic salts) can enhance the binding capacity of the resin, aid in protein solubility and in some cases improve the selectivity of the HIC resin being used. These factors are critical to success in process chromatography applications and this strategy of combining different salts in HIC will be discussed. In Part II of this talk, Mixed-Mode Chromatography (MMC) often employs HIC as one of the modes utilized which enhances the selectivity. MMC, therefore, can be thought of as a logical extension to HIC. MMC often has even higher capacities with unique selectivities when compared to HIC resins. The newly introduced TOYOPEARL MX-Trp-650M resin has very high capacity and excellent salt tolerance under common mixed-mode chromatographic conditions. These unique features along with some of the other properties of TOYOPEARL MX-Trp-650M will be discussed.P>
Presented by: J. Kevin O’Donnell, Ph.D.
Technical Service Manager Process Chromatography, Tosoh Bioscience LLC
