NIAID-sponsored Pathogen Functional Genomic Resource Center, J. Craig Venter Institute

1. PFGRC Gateway® Cloning Survey - July 2008

We greatly appreciate feedback from investigators who are using (or plan to use) our Invitrogen Gateway® entry clones and ask that you take a few moments to fill out the survey below. Such valuable feedback will enable us to improve our existing service and extend new services to the pathogen research community. This survey is completely anonymous and requires nothing from you but a little time. If you would like to offer suggestions or feedback beyond this survey please e-mail PFGRC@jcvi.org

If you have not recently visited our website and clone ordering page, you can do so at this address: http://pfgrc.jcvi.org/index.php/gateway_clones

Thank you.

1. How did you hear about our Gateway® entry clone resource?

How did you hear about our Gateway® entry clone resource? Word of mouth

Website

Scientific meeting

Print advertisement

Other (please specify)

2. What type(s) of entry clones have you ordered from the PFGRC in the past?

What type(s) of entry clones have you ordered from the PFGRC in the past? Custom clones

Whole genome clone set

Both

Have not ordered before

In what year(s) did you order clones?

3. What criteria motivated your clone selection?

What criteria motivated your clone selection? Organism

Strain

Specific list of gene loci

Functional role category

Common name

Gene family

Orthology across species

Metabolic pathways

Protein complexes

Other searchable criteria (please list)

4. Did you find the current search feature useful when ordering?

Did you find the current search feature useful when ordering? Yes

Did not need to use

Did not know about this feature

Suggestions to make the search feature better?

5. Would you use the following features of an enhanced Gateway® clone ordering website?

	Definitely would not	Probably would not	Do not know	Probably would	Definitely would
Look up a clone by locus name	Would you use the following features of an enhanced Gateway® clone ordering website? Look up a clone by locus name Definitely would not	Probably would not	Do not know	Probably would	Definitely would
Upload a file of locus names	Upload a file of locus names Definitely would not	Probably would not	Do not know	Probably would	Definitely would
Search common name text	Search common name text Definitely would not	Probably would not	Do not know	Probably would	Definitely would
Search by nucleotide sequence	Search by nucleotide sequence Definitely would not	Probably would not	Do not know	Probably would	Definitely would
Search by GI number	Search by GI number Definitely would not	Probably would not	Do not know	Probably would	Definitely would
Search by EC number	Search by EC number Definitely would not	Probably would not	Do not know	Probably would	Definitely would
Search by GO term	Search by GO term Definitely would not	Probably would not	Do not know	Probably would	Definitely would
Search by gene symbol	Search by gene symbol Definitely would not	Probably would not	Do not know	Probably would	Definitely would
Cross-homology search (BLAST)	Cross-homology search (BLAST) Definitely would not	Probably would not	Do not know	Probably would	Definitely would

Suggestions for other clone selection features?

6. How would you rate us on the following criteria:

	poor	fair	good	excellent	N/A
Website ease of use	How would you rate us on the following criteria: Website ease of use poor	fair	good	excellent	N/A
Quality of information on website	Quality of information on website poor	fair	good	excellent	N/A
Communication from production lab	Communication from production lab poor	fair	good	excellent	N/A
Communication from legal dept.	Communication from legal dept. poor	fair	good	excellent	N/A
Turnaround time (6-8 week standard)	Turnaround time (6-8 week standard) poor	fair	good	excellent	N/A
Professionalism	Professionalism poor	fair	good	excellent	N/A
Quality of clones	Quality of clones poor	fair	good	excellent	N/A

What additional cloning services would be useful?

7. What features are important for downstream applications?

	Yes	No	No preference
Absent stop codon for COOH-terminal protein fusions	What features are important for downstream applications? Absent stop codon for COOH-terminal protein fusions Yes	No	No preference
Native stop codons	Native stop codons Yes	No	No preference
Altered stop codons	Altered stop codons Yes	No	No preference
Standardized start codon (ATG)	Standardized start codon (ATG) Yes	No	No preference
Ability to order sub-gene fragments	Ability to order sub-gene fragments Yes	No	No preference
Ability to order knock-down (antisense expression) clones	Ability to order knock-down (antisense expression) clones Yes	No	No preference

Please describe your downstream applications (include any expression vectors)?

8. Would you order clones if available in protein expression vectors (as opposed to entry vector)?

Would you order clones if available in protein expression vectors (as opposed to entry vector)? Yes

9. What type of expression vectors would be of greatest interest?

What type of expression vectors would be of greatest interest? Bacterial

Insect Cell

Mammalian

Other (please specify)

10. Would you be interested in knock-out/knock-in clones if they were made available?

Would you be interested in knock-out/knock-in clones if they were made available? Yes

If No, please explain your reason (e.g. model system does not support targeted recombination)

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