Serological and molecular techniques for the detection of plant pathogens are being developed by Ohio State University faculty.

In order bring to market quality, diagnostic assay kits that best satisfy the needs of diagnostic labs and their customers, we are requesting a few minutes of your time to provide us with your input.

1. Does your laboratory provide diagnostic testing which utilizes serology to detect plant pathogens?

Does your laboratory provide diagnostic testing which utilizes serology to detect plant pathogens? Yes

2. If yes, what are the primary companies from which you purchase serological diagnostic kits?

If yes, what are the primary companies from which you purchase serological diagnostic kits? Name of Firm
Location
Name of Firm
Location

3. Does your laboratory provide diagnostic testing which utilizes PCR to detect plant pathogens?

Does your laboratory provide diagnostic testing which utilizes PCR to detect plant pathogens? Yes

4. If yes, what are the primary companies from which you purchase PCR diagnostic kits?

If yes, what are the primary companies from which you purchase PCR diagnostic kits? Name of Firm
Location
Name of Firm
Location

5. Please provide relevant contact information for your laboratory below:

Please provide relevant contact information for your laboratory below: Laboratory Name
Address
City
State
Country
Postal Code
Name of staff member responsible for purchasing molecular and/or serological assay kits.
Email Address

6. Do you currently offer testing for phytoplasmas (mycoplasma-like organisms)?

Do you currently offer testing for phytoplasmas (mycoplasma-like organisms)? Yes

7. Serological?

Serological? Yes

8. PCR?

PCR? Yes

9. Have you experienced any major problems or issues with this testing?

Have you experienced any major problems or issues with this testing? Yes

10. Please explain (from question #9):

Please explain (from question #9):

11. Do you perceive a need for an assay to detect phytoplasmas (mycoplasma-like organisms)?

	PCR?	Serological lab kit?	Serological field kit?
Yes	Do you perceive a need for an assay to detect phytoplasmas (mycoplasma-like organisms)? Yes PCR?	Serological lab kit?	Serological field kit?
No	No PCR?	Serological lab kit?	Serological field kit?

12. Would a general assay kit that detects all phytoplasmas be used by your laboratory?

Would a general assay kit that detects all phytoplasmas be used by your laboratory? Yes

13. Would a specific phytoplasma detection kit (assay that detects members of a specific group, e.g. aster yellows) be used by your laboratory?

Would a specific phytoplasma detection kit (assay that detects members of a specific group, e.g. aster yellows) be used by your laboratory? Yes

14. Please name the phytoplasmas for which you would utilize a GENERAL assay kit.

Please name the phytoplasmas for which you would utilize a GENERAL assay kit.

15. Please name the phytoplasmas for which you would utilize a SPECIFIC assay kit.

Please name the phytoplasmas for which you would utilize a SPECIFIC assay kit.

16. What is the likelihood that your lab would purchase these kits if available in the near future from a reputable firm?

What is the likelihood that your lab would purchase these kits if available in the near future from a reputable firm? Very likely

It’s possible

Not likely (see 17)

17. What is the reason that it would be unlikely for you to purchase phytoplasma assay kits?

What is the reason that it would be unlikely for you to purchase phytoplasma assay kits? PCR?
Serological?

18. Please estimate the annual quantity of phytoplasma assays your lab would run. (assays/yr.)

Please estimate the annual quantity of phytoplasma assays your lab would run. (assays/yr.) Laboratory PCR
Serological lab kit
Serological field kits

19. Approximately what price range would make it feasible for you to purchase these assays when available (if applicable)?

Approximately what price range would make it feasible for you to purchase these assays when available (if applicable)? Laboratory PCR: $
to $
Serological Lab Kit: $
to $
Serological Field Kits: $
to $

20. If you have any questions or have comments to share, please feel free to utilize the space below. We appreciate your time and valuable input.

If you have any questions or have comments to share, please feel free to utilize the space below. We appreciate your time and valuable input.

THANK YOU!
Karen Germ, Business Development
Ohio State University Center for Diagnostic Assays
germ.4@osu.edu

PHYTOPLASMA DIAGNOSTICS SURVEY