Biodiscovery Chromatography Challenges Question Title * 1. How would you best describe your work? (Select all that apply) Basic Research/Biodiscovery Preclinical studies Clinical development Manufacturing Question Title * 2. What type of organization do you work for? Contract Research Organization (CRO) Pharmaceutical/Biotech Company Academic Institution Antibody/Protein Supplier Government Institution Other (please specify) Question Title * 3. What is the size of your company? Less than 50 employees 50 to 100 employees 100 to 500 employees 500 to 2,000 employees More than 2,000 employees Question Title * 4. What is your job function? Scientist in clinical research Scientist in basic research Process development engineer/chemist Lab manager R&D director Sales and marketing Supply chain Other (please specify) Question Title * 5. How often do you use the following chromatography techniques? Daily Weekly Monthly Rarely N/A Affinity Affinity Daily Affinity Weekly Affinity Monthly Affinity Rarely Affinity N/A Ion Exchange Ion Exchange Daily Ion Exchange Weekly Ion Exchange Monthly Ion Exchange Rarely Ion Exchange N/A Size Exclusion Size Exclusion Daily Size Exclusion Weekly Size Exclusion Monthly Size Exclusion Rarely Size Exclusion N/A HIC HIC Daily HIC Weekly HIC Monthly HIC Rarely HIC N/A Mixed Mode Mixed Mode Daily Mixed Mode Weekly Mixed Mode Monthly Mixed Mode Rarely Mixed Mode N/A Reversed Phase Reversed Phase Daily Reversed Phase Weekly Reversed Phase Monthly Reversed Phase Rarely Reversed Phase N/A Immobilized Metal Affinity Chromatography (IMAC) Immobilized Metal Affinity Chromatography (IMAC) Daily Immobilized Metal Affinity Chromatography (IMAC) Weekly Immobilized Metal Affinity Chromatography (IMAC) Monthly Immobilized Metal Affinity Chromatography (IMAC) Rarely Immobilized Metal Affinity Chromatography (IMAC) N/A Question Title * 6. What is the typical instrument pressure you are working at? Less than 0.5 bar 0.5 - 3 bar 3 - 5 bar Greater than 5 bar Question Title * 7. Assuming you had an FPLC column that could withstand the maximum pressure of your existing system, would you consider working at higher pressures in order to improve productivity? (Select all that apply) No Yes, but need to consider loss in dynamic capacity Yes, but need to consider loss in resolution Yes, no issue at all Question Title * 8. If no, why? Question Title * 9. What scale (material to purify) do you typically need for your research purposes? 1 ug - 1mg 1mg - 50mg 50mg - 200mg 200mg - 750mg Greater than 750mg Question Title * 10. How often do you use the following size columns? Always Frequently Sometimes Never 1mL 1mL Always 1mL Frequently 1mL Sometimes 1mL Never 5mL 5mL Always 5mL Frequently 5mL Sometimes 5mL Never 10-15mL 10-15mL Always 10-15mL Frequently 10-15mL Sometimes 10-15mL Never 20-25mL 20-25mL Always 20-25mL Frequently 20-25mL Sometimes 20-25mL Never Greater than 25mL Greater than 25mL Always Greater than 25mL Frequently Greater than 25mL Sometimes Greater than 25mL Never Question Title * 11. How many columns do you use in one year? 1 – 50 columns 50-100 columns 100-250 columns 250-500 columns Greater than 500 columns Question Title * 12. How many batches of antibody do you purify per month? None 1-5 batches 5-10 batches Greater than 10 batches Question Title * 13. What is the average number of purification cycles you perform in a month? 1-5 purification cycles 5-10 purification cycles 10-20 purification cycles Greater than 20 purification cycles Question Title * 14. At what point do you perform clean-in-place (CIP) on the column? After every cycle After 5 cycles After 10 cycles After more than 10 cycles Never Question Title * 15. Indicate the importance level for each factor below in your decision to use a particular FPLC column (where 5 is very important and 1 is not important at all) 1 (not important at all) 2 3 4 5 (very important) Price Price 1 (not important at all) Price 2 Price 3 Price 4 Price 5 (very important) Product lifetime Product lifetime 1 (not important at all) Product lifetime 2 Product lifetime 3 Product lifetime 4 Product lifetime 5 (very important) Reliability Reliability 1 (not important at all) Reliability 2 Reliability 3 Reliability 4 Reliability 5 (very important) High pH stability High pH stability 1 (not important at all) High pH stability 2 High pH stability 3 High pH stability 4 High pH stability 5 (very important) Ease of use Ease of use 1 (not important at all) Ease of use 2 Ease of use 3 Ease of use 4 Ease of use 5 (very important) Dynamic binding capacity Dynamic binding capacity 1 (not important at all) Dynamic binding capacity 2 Dynamic binding capacity 3 Dynamic binding capacity 4 Dynamic binding capacity 5 (very important) Selectivity Selectivity 1 (not important at all) Selectivity 2 Selectivity 3 Selectivity 4 Selectivity 5 (very important) Product availability (supply chain) Product availability (supply chain) 1 (not important at all) Product availability (supply chain) 2 Product availability (supply chain) 3 Product availability (supply chain) 4 Product availability (supply chain) 5 (very important) Vendor familiarity Vendor familiarity 1 (not important at all) Vendor familiarity 2 Vendor familiarity 3 Vendor familiarity 4 Vendor familiarity 5 (very important) Vendor technical support Vendor technical support 1 (not important at all) Vendor technical support 2 Vendor technical support 3 Vendor technical support 4 Vendor technical support 5 (very important) Other Other 1 (not important at all) Other 2 Other 3 Other 4 Other 5 (very important) Question Title * 16. If other was selected, please specify. Question Title * 17. Indicate the improvement you feel is needed for the following areas. Desperately Needs Improvement Needs Some Improvement No Improvement Needed N/A Cost of consumables Cost of consumables Desperately Needs Improvement Cost of consumables Needs Some Improvement Cost of consumables No Improvement Needed Cost of consumables N/A Cycle time Cycle time Desperately Needs Improvement Cycle time Needs Some Improvement Cycle time No Improvement Needed Cycle time N/A Reduce number of cycles Reduce number of cycles Desperately Needs Improvement Reduce number of cycles Needs Some Improvement Reduce number of cycles No Improvement Needed Reduce number of cycles N/A Eliminate CIP Eliminate CIP Desperately Needs Improvement Eliminate CIP Needs Some Improvement Eliminate CIP No Improvement Needed Eliminate CIP N/A Product lifetime Product lifetime Desperately Needs Improvement Product lifetime Needs Some Improvement Product lifetime No Improvement Needed Product lifetime N/A Question Title * 18. Where do you experience the biggest bottleneck in your process? Upstream process Speed of loading Elution volume Regeneration and CIP Purchasing and procurement I don't have any bottlenecks Other (please specify) Question Title * 19. Assuming you pay $200 for a one 1mL affinity FPLC column, what would be the price point you would consider for a single use, 1mL disposable column? Question Title * 20. Who makes the purchase decision on what FPLC product (consumable) you use? Process scale department Third-party partner Lab manager Individual user Other (please specify) Question Title * 21. What type of companies do you typically purchase from? (Select all that apply) Large global distributor (ex: Thermo-Fisher, VWR) Direct from the manufacturer Local distributor Instrument company Other (please specify) Question Title * 22. How does your company purchase chromatography supplies? (Select all that apply) Individuals purchase with company credit/purchase cards Central purchasing department places a purchase order Web store From the company stockroom Contracted distributor program Method of purchase depends on value of order Question Title * 23. In order to be included in our drawing for a $100 Amazon gift card (two available), please enter your contact information below. Name Company Address Address 2 City/Town State/Province ZIP/Postal Code Country Email Address Thank you for taking the time to provide this valuable insight. We truly appreciate it!- The Life Science Connect Team Submit
